My current research focuses on exploring different aspects of translation regulation as well as protein degradation pathways in the minimal cell model M. pneumoniae. As a member of the team of the MycoSynVac project, I am also involved in the design of a mycoplasma chassis optimized to grow faster in bioreactors, a requirement to create a cost-effective product in industry. To do so, we are rationally modifying its genome in combination with the establishment of a serum-free medium.
Publications
, 2022. SURE editing: combining oligo-recombineering and programmable insertion/deletion of selection markers to efficiently edit the Mycoplasma pneumoniae genome. Nucleic Acids Res.
, 2021. Widespread ribosome stalling in a genome-reduced bacterium and the need for translational quality control. iScience 24(9):102985
, 2020. Protein quality control and regulated proteolysis in the genome-reduced organism Mycoplasma pneumoniae. Mol Syst Biol 16(12):e9530
, 2020. Model-driven design allows growth of Mycoplasma pneumoniae on serum-free media. NPJ Syst Biol Appl 6(1):33
, 2020. FASTQINS and ANUBIS: two bioinformatic tools to explore facts and artifacts in transposon sequencing and essentiality studies. Nucleic Acids Res 48(17):e102
, 2020. Impact of C-terminal amino acid composition on protein expression in bacteria. Mol Syst Biol 16(5):e9208
, 2018. Mycoplasma genitalium Nonadherent Phase Variants Arise by Multiple Mechanisms and Escape Antibody-Dependent Growth Inhibition. Infect Immun 86.
, 2014. Characterization of the operon encoding the Holliday junction helicase RuvAB from Mycoplasma genitalium and its role in mgpB and mgpC gene variation. J Bacteriol 196(8):1608-18
, 2014. MG428 is a novel positive regulator of recombination that triggers mgpB and mgpC gene variation in Mycoplasma genitalium. Mol Microbiol 94(2):290-306
, 2012. RecA mediates MgpB and MgpC phase and antigenic variation in Mycoplasma genitalium, but plays a minor role in DNA repair. Mol Microbiol 85(4):669-83
, 2009. P110 and P140 cytadherence-related proteins are negative effectors of terminal organelle duplication in Mycoplasma genitalium. PLoS One 4(10):e7452
, 2008. Role of Mycoplasma genitalium MG218 and MG317 cytoskeletal proteins in terminal organelle organization, gliding motility and cytadherence. Microbiology (Reading) 154(Pt 10):3188-3198
, 2008. Deletion of the Mycoplasma genitalium MG_217 gene modifies cell gliding behaviour by altering terminal organelle curvature. Mol Microbiol 69(4):1029-40
, 2007. Functional analysis of the Mycoplasma genitalium MG312 protein reveals a specific requirement of the MG312 N-terminal domain for gliding motility. J Bacteriol 189(19):7014-23
, 2006. Mycoplasma genitalium P140 and P110 cytadhesins are reciprocally stabilized and required for cell adhesion and terminal-organelle development. J Bacteriol 188(24):8627-37
, 2006. Mycoplasma genitalium mg200 and mg386 genes are involved in gliding motility but not in cytadherence. Mol Microbiol 60(6):1509-19
, 2006. Comparative analysis of antibiotic resistance gene markers in Mycoplasma genitalium: application to studies of the minimal gene complement. Microbiology (Reading) 152(Pt 2):519-527
Publication list retrieved from NCBI using ImpactPubs
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